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(More customer reviews)This is by and large one of the best compendiums of cDNA library generation protocols that I have seen. Detailed protocols for numerous aspects, outlining helpful tips and potential pitfalls. Highly recommended for any lab doing cDNA libraries.
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Expert researchers and inventors in the field describe their own proven techniques for generating cDNA/mRNA libraries to identify the functions of specific decoded gene sequences. A wide variety of techniques is presented for enhancing the generation of complete and full-length libraries, and for confirming the quality of the cDNAs generated. Among the applications detailed are electrophoresis, Northern blotting, single cell microarray analysis, subtractive hybridization, subtractive cloning, gene cloning, and peptide library generation.
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